MicroRNA-195 regulates biological behavior of hemangioma cell lines by targeting HIF-1a

MicroRNAs (miRNAs) have recently been reported play a crucial role in some tumors. In order to investigate the association of miR-195 with hemangioma, we investigate the expression of miR-195 in hemangioma tissues and its role in biological behavior of XTPS-1 and HemEChemangioma cancer cell lines. Quantitative qPCR results showed miR-195 was significantly down-regulated in hemangioma tissues compared with normal tissues. Over-expression of miR-195 was established by transfecting miR-195 mimics into XTPS-1 and HemECs cells, and its effects on the biological behavior of hemangioma cancer were studied using transwell assay, MTT and flow cytometry. Ectopic expression of miR-195 promoted cell proliferation of hemangioma cancer cells, while loss-of-function assay showed the opposite effect. Results indicated up-regulation of miR-195 significantly inhibited cell proliferation, invasion and resulted in apoptosis of the hemangioma cancer cells. Moreover, we demonstrated that miR-195 down-regulated HIF-1a expression by directly targeting the HIF-1a 3’-untranslated region. Thus, our findings suggested that miR-195 acted as a new tumor suppressor by targeting the HIF-1a gene and inhibiting cell proliferation and invasion of hemangioma.